One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.

CDX-2 (EPR2764Y) Rabbit Monoclonal Antibody is intended for laboratory use in the detection of the CDX-2 protein in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This antibody is intended for in vitro diagnostic (IVD) use.

CDX-2 (EPR2764Y) Rabbit Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.

CDX-2 is a caudal-related homeobox transcription factor that plays a role in the development and maintenance of intestinal mucosa. This protein is expressed in normal and neoplastic gastrointestinal epithelium, including colorectal adenocarcinoma. It is useful for identifying gastrointestinal origin of benign and malignant lesions.^1-7

1. Mazziotta, Robert M et al. “CDX2 immunostaining as a gastrointestinal marker: expression in lung carcinomas is a potential pitfall.” Applied immunohistochemistry & molecular morphology : AIMM vol. 13,1 (2005): 55-60.
2. Erickson, Lori A et al. “Cdx2 as a marker for neuroendocrine tumors of unknown primary sites.” Endocrine pathology vol. 15,3 (2004): 247-52.
3. Saqi, Anjali et al. “Usefulness of CDX2 and TTF-1 in differentiating gastrointestinal from pulmonary carcinoids.” American journal of clinical pathology vol. 123,3 (2005): 394-404.
4. Saad, Reda S et al. “Usefulness of Cdx2 in separating mucinous bronchioloalveolar adenocarcinoma of the lung from metastatic mucinous colorectal adenocarcinoma.” American journal of clinical pathology vol. 122,3 (2004): 421-7.
5. Kaimaktchiev, Vassil et al. “The homeobox intestinal differentiation factor CDX2 is selectively expressed in gastrointestinal adenocarcinomas.” Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc vol. 17,11 (2004): 1392-9.
6. Werling, Robert W et al. “CDX2, a highly sensitive and specific marker of adenocarcinomas of intestinal origin: an immunohistochemical survey of 476 primary and metastatic carcinomas.” The American journal of surgical pathology vol. 27,3 (2003): 303-10.
7. Moskaluk, Christopher A et al. “Cdx2 protein expression in normal and malignant human tissues: an immunohistochemical survey using tissue microarrays.” Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc vol. 16,9 (2003): 913-9.

EPR2764Y

Rabbit

IgG₁/K

Nucleus

Upon receipt and when not in use, store at 2-8°C. Do not freeze.

Colon Adenocarcinoma

Normal Colon