One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.

Myogenin (F5D) Mouse Monoclonal Primary Antibody is intended for laboratory use in the detection of the myogenin protein in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments.

This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.

This antibody is intended for in vitro diagnostic (IVD) use.

Myogenin (F5D) Mouse Monoclonal Antibody (this antibody) may be used as the primary
antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue
sections. In general, immunohistochemical staining allows the visualization of antigens
via the sequential application of a specific antibody (primary antibody) to the antigen, a
secondary antibody (link antibody) to the primary antibody, an enzyme complex and a
chromogenic substrate with interposed washing steps. The enzymatic activation of the
chromogen results in a visible reaction product at the antigen site. The specimen may then be
counterstained and a coverslip applied. Results are interpreted using a light microscope and
aid in the differential diagnosis of pathophysiological processes, which may or may not be
associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.

Myogenin also identified as myogenic factor 4 is a muscle specific transcription factor associated with muscle differentiation and cell cycle.¹ Anti-myogenin reactivity is seen in the nuclei of myoblasts in developing muscle tissue. Anti-myogenin is a useful immunohistochemical reagent for identification of rhabdomyosarcoma.^2-5

1. Miller JB. Myogenic programs of mouse muscle cell lines: expression of myosin heavy chain isoforms, MyoD1, and myogenin. J Cell Biol. 1990; 111:1149-59.
2. Wang NP, et al. Expression of myogenic regulatory proteins (myogenin and MyoD1) in small blue round cell tumors of childhood. Am J Pathol. 1995; 147:1799-810.
3. Cui S, et al. Evaluation of new monoclonal anti-MyoD1 and anti-myogenin antibodies for the diagnosis of rhabdomyosarcoma. Pathol Int. 1999; 49:62-8.
4. Kaspar et al. The expression of c-Myb correlates with the levels of rhabdomyosarcomaspecific marker myogenin. Sci Rep. 2015; 5:15090.
5. Rudzinski et al. Myogenin, AP 2 Beta, NOS-1 and HMGA2 are surrogate markers of fusion status in rhabdomyosarcoma. Am J Surg Pathol. 2014; 38:654-9.

F5D

Mouse

IgG₁/K

Nucleus

Upon receipt and when not in use, store at 2-8°C. Do not freeze.

Rhabdomyosarcoma